Estimates of costs of archiving biological samples
Data submitted by workshop attendees in 2003 are shown in Table 1 and summarized below.
- Staff time per sample is generally in the range of 10 to 20 minutes. A sample (or lot) consists of specimens of a single taxon, usually a species, that resulted from a single collecting event.
- Material costs range from $0.20 to $3.00 per sample. This depends largely on the size and number of specimens in the samples. In the table, cost is for a single vial unless otherwise stated. Tissue and frozen samples are less expensive on a per vial basis because of their small size, but if sample is split into aliquots, costs of supplies and storage increase linearly, but labor costs per vial decrease.
- Equipment costs for dry and wet collections depend on the typical body size of taxa and on how the collection is housed (standard steel shelving versus custom-made cabinets; compactorized or not). Range was $0.22 to $3.00 per sample.
- Maintenance costs of samples range from negligible to $0.17 per sample per year. This includes staff time to check the integrity of samples (for example topping off fluid in ethanol perserved collections), service contracts on equipment, and, for -80°C collections, periodic replacement of compressors and freezers. In general, dry collections (slide, whole specimens, or freeze-dried tissues) were least expensive, then fluid, then frozen at -80°C, then storage in liquid nitrogen.
- Cost of maintenance of space, including climate control, lighting, cleaning, repairs, etc., was $80 to $110 per square meter per year for the three institutions that could provide this information. For the purposes of estimating costs, $100 per square meter per year is reasonable.
- Total costs to house specimen collections in perpetuity range from about $5 to $24 per sample (excluding marine mammals and histological preparation of flatworms). This is based on labor costs of $25/hour, and multiplies annual costs by 20, which approximates a permanent endowment yielding 5% income.
Table 1. |
Estimates of costs of archiving by taxon and method. For freeze-drying, maintenance is at -4°C or -20°C. Items in square brackets were not provided originally, but estimated for the purpose of calculating total cost. |
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Sample type |
Method |
Labor (minutes/ sample) |
Supplies ($/sample) |
Equipment ($/sample) |
Annual maintenance ($/sample) |
Samples/ sq. m. |
Total per sample |
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Tissue |
Freezing, -80°C |
[15]
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0.25
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0.52
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0.03
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[4000]
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$8.12
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Liquid nitrogen |
[15]
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0.25
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0.85
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0.17
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[4000]
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$11.25
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Freeze drying |
[15]
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0.25
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0.28
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0.006
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[4000]
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$6.90
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Flatworms |
Formalin/ethanol |
20
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0.40
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0.25
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0.10
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[2000]
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$11.98
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Ethanol |
20
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0.40
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0.25
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0.10
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[2000]
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$11.98
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Slides |
180
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2.00
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0.01
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0
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10000
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$75.21
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Mollusks |
Dry |
10
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0.25
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0.80
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0.01
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1200
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$7.21
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Ethanol |
15
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1.30
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0.22
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0.04
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900
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$10.97
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Freezing, -80°C |
15
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0.30
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0.60
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0.03
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2000
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$8.75
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Cnidarians |
Dry |
10
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0.32
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1.88
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[0.01]
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150
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$19.9
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Formalin/ethanol |
10
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1.23
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3.00
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[0.10]
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150
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$23.73
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Ethanol |
10
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1.44
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3.00
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[0.10]
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150
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$23.94
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Freezing, -80°C |
10
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0.90
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[.60]
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[0.03]
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1000
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$8.27
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Crustacea |
Formalin/ethanol |
20
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2.90
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0.86
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[.10]
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[1000]
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$16.09
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Ethanol |
15
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2.40
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0.86
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[.10]
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[1000]
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$13.51
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Polychaetes |
Formalin/ethanol |
10-15
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0.85
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0.06
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0.002
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20000
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$7.30
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Ethanol |
5-10
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0.77
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0.06
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0.002
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20000
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$5.14
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Freezing, -80°C |
5-10
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0.77
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1.75
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0.09
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4500
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$8.93
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Macro-inverts |
Dry |
15
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0.35
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[.80]
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[0.01]
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1500
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$8.93
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Formalin/ethanol |
15
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1.20
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[.80]
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0.05
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1200
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$10.92
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Ethanol |
15
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1.20
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[.80]
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0.05
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1200
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$10.92
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Fish (whole) |
Formalin/ethanol |
15
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0.80
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2.00
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0.15
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320
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$18.3
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Fish (tissue) |
Ethanol |
15
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0.20
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0.75
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0.04
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3900
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$8.51
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